Anti-phospho-TrkB (Tyr816) Antibody (C15-1317-354)

General description

BDNF/NT-3 growth factors receptor (EC 2.7.10.1; UniProt Q63604; also known as GP145-TrkB/GP95-TrkB, Trk-B, TrkB tyrosine kinase, Neurotrophic tyrosine receptor kinase type 2, Neural receptor protein-tyrosine kinase) is encoded by the Ntrk2 gene (also known as RATTRKB1, Trkb, TRKB1) in rat (Gene ID 25054). Neurotrophins mediate a wide range of neuronal functions, including survival, differentiation, growth, and death, via two classes of transmembrane receptors, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor. While nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and NT-4 all bind the p75 receptor, TrkA is selective for NGF, TrkB is selective for BDNF and NT-4, and TrkC is selective for NT-3. TrkA and TrkB receptors can also be activated by ligands of the G-protein-coupled receptor (GPCRs), including the nucleoside adenosine or adenosine agonists, such as CGS 21680 and the pituitary adenylate cyclase-activating polypeptide (PACAP). In addition to transactivation via GPCRs and glucocorticoids are also reported to promote TrkA/B/C activation due to signaling cross-talk between Trk and GC receptor (GR).

Specificity

This polyclonal antibody selectively detects BDNF-induced pTyr816 phosphorylation of both glycosylated (145 kDa) and unprocessed (110 kDa) TrkB in primary embryonic rat E18 hippocampal neurons, but not NGF-induced pTyr490 or pTyr794 phosphorylation of TrkA in primary embryonic septal neurons (PMID 18347336).

Immunogen

Epitope: Near C-terminus

Linear peptide corresponding to rat TrkB sequence near the C-terminus with phosphorylated Tyr816.

Application

Detect phospho-TrkB (Tyr816) using this Anti-phospho-TrkB (Tyr816) Antibody validated for use in Western Blotting, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry.

Immunocytochemistry Analysis: A representative lot detected BDNF- and adenosine-stimulated TrkB Tyr816 phosphorylation in cultured hippocampal neurons (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Tyr816 phosphorylated TrkB from BDNF-stimulated HEK293 cells expressing transfected TrkB (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University).
Western Blotting Analysis: A representative lot detected dexamethasone-(Dex-) and BDNF-induced TrkB Tyr816 phosphorylation in cortical slices from postnatal P9–P10 rats, as well as in dorsal striatum from P18-20 rats received intraperitoneal Dex injection (Jeanneteau, F., et al. (2008). Proc Natl Acad Sci USA. 105(12):4862-4867).
Western Blotting Analysis: A representative lot detected TrkB Tyr816 phosphorylation in mice brain synaptosome preparations (Parkhurst, C.N., et al. (2013). Cell. 155(7):1596-1609).
Western Blotting Analysis: A representative lot detected BDNF-stimulated TrkB Tyr816 phosphorylation in cultured mouse hippocampal neurons and HEK293 cells expressing murine TrkB (Bath, K.G., et al. (2008). J. Neurosci. 28(10):2383-2393).
Immunohistochemistry Analysis: A representative lot detected enhanced TrkB Tyr816 phosphorylation in the dentate gyrus tissue sections from postnatal P18 rats after receiving metyrapone and/or dexamethasone treatment (Jeanneteau, F., et al. (2008). Proc Natl Acad Sci USA. 105(12):4862-4867).
Immunohistochemistry Analysis: A representative lot detected TrkB Tyr816 phosphorylation in coronal slices of the rostral migratory stream (RMS) from adult mice (Bath, K.G., et al. (2008). J. Neurosci. 28(10):2383-2393).
Immunocytochemistry Analysis: A representative lot detected corticosterone-stimulated TrkB Tyr816 phosphorylation in 5-hour starved rat hippocampal neuron culture (Jeanneteau, F., et al. (2008). Proc Natl Acad Sci USA. 105(12):4862-4867).
Immunocytochemistry Analysis: A representative lot detected BDNF-stimulated TrkB Tyr816 phosphorylation in cultured DRG neurons from E16.5 rat embryos (Arevalo, J.C., et al. (2006). Neuron. 50(4):549-559).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in BDNF treated murine TrkB-transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-TrkB (Tyr816) in 10 µg of BDNF treated murine TrkB-transfected HEK293 cell lysate.

Target description

~140 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat. species reactivity (predicted by homology): gorilla (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), human (based on 100% sequence homology), horse (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_036863. UniProt accession no.: Q63604. shipped in: wet ice. target post-translational modification: phosphorylation (pTyr816 ). Gene Information: human ... NTRK2(4915)
rat ... Ntrk2(25054). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.