ANTI-PHOSPHO-VASP [PSER239] ANTIBODY PRODUCED IN RABBIT AFFINITY ISOLATED ANTIBODY

General description

Monoclonal Anti-WASH1 (mouse IgG1 isotype) is derived from the hybridoma WASH1-27 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human WASH1. VASP (vasodilator-stimulated phosphoprotein) belongs to the family of Ena/VASP actin-regulatory proteins. VASP is localized at highly dynamic membrane regions, focal adhesion sites, lamellipodia protrusions, filopodia tips and along stress fibers.

Immunogen

synthetic peptide containing phosphorylated Ser²³⁹ of human VASP conjugated to KLH. The corresponding sequence is identical in mouse and rat VASP.

Application

Anti-phospho-VASP [pSer239] antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

VASP (vasodilator-stimulated phosphoprotein) is implicated in cell motility and adhesion. VASP is localized at cell matrix and cell-cell contacts and plays an important role in adherens junction formation and stabilization in epithelial cells. VASP is a substrate for cAMP- and cGMP-dependent protein kinases. It is phosphorylated at multiple sites including Ser157, Ser239 and Thr278. cGMP-dependent protein kinase I (cGKI) phosphorylates VASP in a variety of cells, including platelets, fibroblasts and endothelial cells. In platelets, cGMP-mediated phosphorylation of VASP correlates with inhibition of agonist-induced platelet aggregation. Ena/VASP proteins are required for neurite initiation and extension in the developing cortex. VASP has been shown to be required for endothelial barrier function in vivo. Knockout of Ena/VASP proteins in mice leads to increased endothelial permeability causing fatal vascular leakage and haemorrhaging during late embryonic development. In contrast, overexpression of VASP enhances barrier function of endothelial cells in vitro and increases their force generation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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