General description

Phosphatidylinositol-3-phosphate-5-kinase, type 3 (PIP5K3, also known as PIKfyve and FAB1) belongs to a large family of lipid kinases that modify the phosphorylation state of phosphatidylinositol (PtdIns). PIP5K3 has been shown to be localized on early and late endosomes.

Phosphatidylinositol-3-phosphate/phosphatidylinositol-5-kinase, type III (PIP5K3) is a constituent of a trimolecular protein complex. It is a 2089 amino acid protein. The gene encoding it is located on human chromosome 2 and has 41 exons.

Specificity

Anti-PIP5K3 (N-terminal) specifically recognizes human PIP5K3.

Application

Anti-PIP5K3 (N-terminal) antibody produced in rabbit can be used in several immunochemical techniques including immunoblotting.

Biochem/physiol Actions

Phosphatidylinositol-3-phosphate-5-kinase, type 3 (PIP5K3) enzymatic activity is critical for cell morphology and endocytic membrane homeostasis. PIP5K3 acts as a negative regulator of endosome function or regulating endosome-trans-golgi-network (TGN) trafficking. It negatively regulates exocytosis on neurosecretory cells. PIP5K3 regulates the trafficking of IRAP/GLUT4 vesicles to plasma membrane in adipocytes, suggesting that it may play a pleiotropic role in membrane trafficking, including the exocytic pathway.

Phosphatidylinositol-3-phosphate/phosphatidylinositol-5-kinase, type III (PIP5K3) is involved in the synthesis of phosphatidylinositol 3, 5-bisphosphate. It also possesses a protein kinase activity. PIP5K3 regulates specific signaling pathways as well as membrane trafficking. Mutations in the gene encoding it are associated with fleck corneal dystrophy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.