General description
Serine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1.
Specificity
This antibody recognizes the POLO box domain of PLK-4
Immunogen
Epitope: POLO box domain
KLH-conjugated linear peptide corresponding to the POLO box domain of human PLK-4.
Application
Anti-PLK-4 Antibody detects level of PLK-4 & has been published & validated for use in Western Blotting, ICC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Quality
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected PLK-4 in 10 µg of Jurkat cell lysate.
Target description
~95 kDa observed
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, rat, human. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_0055079. UniProt accession no.: O00444. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... PLK4(10733). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABT142