ANTI-PMCA4B CLONE JA3 (C005B-449738)

General description

Plasma membrane calcium-transporting ATPase 4 (UniProt: P23634; also known as EC: 3.6.3.8, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4) is encoded by the ATP2B4 (also known as ATP2B2, MXRA1) gene (Gene ID: 493) in human. PMCA4 is a multi-pass membrane protein that acts as a calcium/calmodulin-regulated and magnesium-dependent enzyme and catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCA4 contains 2 calmodulin binding regions known as subdomain A (aa 1086-1103) and B (aa 1104-1113) and a PDZ domain-binding motif that lies within the last 7 amino acids of the carboxy terminus, this latter is absent in the PMCAa tail. PMCA4 is shown to be a regulator of neuronal nitric-oxide synthase (nNOS) and is suggested to play a structural role in tethering nNOS to a highly compartmentalized domain in the cardiac cell membrane. PMCA4b is quite ubiquitousi is a major PMCA expressed in developing mammary tissue. The kinetics of binding of Ca2+/CaM to PMCA4b is very slow, but remains active for longer periods when compared to PMCA4a. A significantly reduced migration of PCMA4b expressing in B-RAF mutant cells is linked to their reduced metastatic potential. (Ref.: Strehler, EE. (2015). Biochem. Biophys. Res. Commun. 460(1); 26-33; Afronze, T., et al. (2014). J. Biol. Chem.289 (10), 7221-31); Mohanmed TM et al. (2011). J. Biol. Chem. 286(48):41520-29).

Specificity

Clone JA3 detects Plasma membrane calcium-transporting ATPase in human brain tissue. It targets an epitope with in 25 amino acids from the C-terminal region.

Immunogen

Purified plasma membrane calcium-transporting ATPase from human red blood cells.

Application

Anti-PMCA4b, clone JA3, Cat. No. MABN1801, is a highly specific mouse monoclonal antibody that targets PMCA4b and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected PMCA4b in Western Blotting applications (Caride, A.J., et. al. (1996). Biochem J. 316 (Pt 1):353-9; Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31; DeMarco, S.J., et. al. (2002). J Biol Chem. 277(12):10506-11; Gros, R., et. al. (2003). Circ Res. 93(7):614-21; Paszty, K., et. al. (2015). Sci Signal. 8(364):ra19; Usachev. M., et. al. (2001). J Neurochem. 76(6):1756-65).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PMCA4b in human cerebral cortex, human kidney, and bovine cornea tissues.

Immunohistochemistry Analysis: A representative lot detected PMCA4b in Immunohistochemistry applications (Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31).

Immunocytochemistry Analysis: A representative lot detected PMCA4b in Immunocytochemistry applications (Usachev. M., et. al. (2002). J Neuron. 33(1):113-22).

ELISA Analysis: A representative lot detected PMCA4b in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 (Pt 1):353-9; Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31).

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PMCA4b in 10 µg of human brain tissue lysate.

Target description

~134 kDa observed; 137.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.