Anti-PPOX (C15-1317-810)

General description

Protoporphyrinogen oxidase (UniProt: P50336; also known as EC: 1.3.3.4, PPO, PPOX) is encoded by the PPOX gene (Gene ID: 5498) in human. Protoporphyrinogen oxidase is a mitochondrial inner membrane enzyme that catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. It is involved in step one of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas and may be present as a monomer or active homodimeric form. Defects in PPOX are linked to variegate porphyria that lead to severe deficiency of this enzyme that result in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver.

Specificity

This rabbit polyclonal antibody detects Protoporphyrinogen oxidase (PPO) in Human and Mouse cells.

Immunogen

Epitope: unknown

Full length recombinant human Protoporphyrinogen oxidase (PPOX).

Application

Anti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected PPOX in undifferentiated (day 0) and maturing (day 3 or 4) mouse erythroleukemia (MEL) cells (Chung, J., et. al. (2015). Sci Signal. 8(372):ra34). and differentiated Mouse erythroleukemia (MEL) cells (Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).

Quality

Evaluated by Western Blotting in lystate from mitochondria isolated from differentiated DS19 cells.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.

Target description

~53 kDa observed; 50.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: unpurified. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. species reactivity (predicted by homology): human (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. L. technique(s): western blot: suitable. isotype: IgG. NCBI accession no.: NP_000300. UniProt accession no.: P50336. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... PPOX(5498). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.