General description
PR domain zinc finger protein 16 (PRDM16) is also called Transcription factor MEL1 (MDS1/EVI1-like gene 1). PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. PRDM16 is expressed in the uterus and kidney. PRDM16 regulates transcription through DNA binding and plays a role in the differentiation of myoblastic precursors into brown adipose tissue (BAT), and represses TGF-beta signalling. Translocations in PRDM16 occur in subsets of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) cases.
Specificity
This antibody can recognize all four isoforms of PRDM16.
Immunogen
KLH-conjugated linear peptide corresponding to human PRDM16.
Application
Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PRDM16 in rat brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected PRDM16 in human brain cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 15 µg of rat brain tissue lysate.
Target description
~100 kDa, ~135 kDa and ~150 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms between ~120 kDa and ~140 kDa
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q9HAZ2. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... PRDM16(63976). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABD130