ANTI-PRECEREBELLIN ANTIBODY (C005B-241346)

General description

Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q. Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release. The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus. Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin possess functions other than those already stated.

Immunogen

KLH-conjugated linear peptide corresponding to human Precerebellin.

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Precerebellin in human cerebellum tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Precerebellin antibody is validated for use in WB & IHC for the detection of the Precerebellin protein.

Quality

Evaluated by Western Blot in mouse cerebellum lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Precerebellin in 10 µg/mL of mouse cerebellum tissue lysate.

Target description

~35 kDa observed. The calculated molecular weight is 22 kDa, however, the full length Precerebellin has been shown as a ~35 kDa band. (Bao, D., et al. (2005). Journal of Neurochemistry. 95:618-629.) An uncharacterized band may appear at ~50 kDa in some lysates.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse cerebellum tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. technique(s): immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: P23435. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CBLN1(869). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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