Anti-PREX2 antibody produced in rabbit (C15-1448-311)

General description

PREX2 (phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2) is a homolog of PREX1, and belongs to the Dbl (Diffuse B-cell lymphoma) family guanine nucleotide exchange factors (GEFs) family. It contains a PH (pleckstrin homology) domain, which is essential for substrate recognition. It also contains DH (Dbl-homology) domain and PDZ domains. This protein has a molecular weight of 183kDa.

Immunogen

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

PREX2 (phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2) interacts with and suppresses PTEN (phosphatase and tensin homolog) protein in breast cancer. Mutations in this gene are linked to human melanomas, and abnormal expression of this gene leads to tumorigenesis in immortal melanocytes. Mutations in this gene are also associated with lung, colon and pancreatic cancer. Thus, mutations are thought to turn PREX2 in an oncogene. It recognizes and interacts with Rac-1 protein, RhoA and Cdc42, and activates them. This, in turn, is activated by PI-3K (Phosphoinositide 3-kinase). It is induced by CXCL9 (Chemokine (C-X-C Motif) Ligand 9), which results in enhanced invasiveness of hepatocellular carcinoma. PREX2 is also up-regulated in gastric cancer (GC), and this expression is suppressed by miR-338-3p, which acts as a tumor suppressor in GC.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST72469.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.