ANTI-PRION PROTEIN CLONE POM2 (C15-1886-174)

General description

Neuroligin-4, X-linked (UniProt: Q8N0W4; also known as Neuroligin X, HNLX) is encoded by the NLGN4X (also known as KIAA1260, NLGN4, UNQ365/PRO701) gene (Gene ID: 57502) in human. NLGN4X is a single-pass type I membrane, homodimeric, glycoprotein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 42-676) a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). It is predominantly expressed in the heart tissue. It is also expressed at lower levels in the liver, skeletal muscle, pancreas, and brain. It is a putative neuronal cell surface protein that is involved in cell-cell-interactions. In human, NLGN4X is localized at excitatory synapses, whereas in mouse NLGN4-like is localized at glycinergic synapses. NLGN4X can be phosphorylated by protein kinase C that enhances excitatory synapses. Mutations in NLGN4X gene have been linked to X-linked Asperger syndrome and Autism. (Ref.: Nguyen, TA., et al. (2020). Front. Synaptic Neurosci. 12; 33; Laumonnier, F., et al. (2004). Am. J. Hum. Genet. 74(3); 552-557).

Specificity

Clone CSTEM30 is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X). It targets an epitope within the extracellular domain.

Immunogen

His-tagged recombinant protein fragment corresponding to the extracellular domain of human Neuroligin-4,x-linked (NLGN4X).

Application

Quality Control Testing

Evaluated by Flow Cytometry in HFF-hiPSCs.

Flow Cytometry Analysis (FC): 1 μg of this antibody detected NLGN4X in one million Human foreskin fibroblast-induced pluripotent stem cells (HFF-hiPSCs) .

Tested applications

Flow Cytometry Analysis: A representative lot detected NLGN4X in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640; Liu, X., et al. (2017). Nat Methods. 14(11):1055-1062; Liu, X., et al. (2020). Nature. 586(7827):101-107).

Immunocytochemistry Analysis: A representative lot detected NLGN4X in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).

ELISA Analysis: A representative lot detected NLGN4X in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-NLGN4X, clone CSTEM30, Cat. No. MABN2301, is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X) and is tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.

Physical form

Purified mouse monoclonal antibody Ig G2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.