Anti-Properdin Antibody (C15-1317-281)

General description

Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.

Specificity

This polyclonal antibody detects properdin by targeting a sequence in the fourth TSP type-1 domain (TSP type-1 domain 3).

Immunogen

Epitope: Within TSP type-1 domain 3.

KLH-conjugated linear peptide corresponding to a TSP type-1 domain 3 sequence of human Properdin.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

This Anti-Properdin Antibody is validated for use in Western Blotting for the detection of Properdin.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Properdin in 10 µg of mouse serum.

Quality

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Properdin in 10 µg of rat serum.

Target description

~52 kDa observed. 48.49/47.94/48.18 kDa (human/mouse/rat mature form) and 51.28/50.33/50.57 kDa (human/mouse/rat pro-form) calculated.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. species reactivity (predicted by homology): nonhuman primates (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_002612. UniProt accession no.: P27918. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CFP(5199). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.