Anti-proSAAS (C15-1317-506)

General description

ProSAAS (UniProt: Q9QXV0; also known as IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS) is encoded by the Pcsk1n gene (Gene ID: 30052) in murine species. ProSAAS is a small secretory protein that is primarily expressed in neural and neuroendocrine tissues and acts as an anti-aggregant chaperone. Its presence appears to be restricted only to vertebrates. Mammalian proSAAS does not undergo either glycosylation or phosphorylation, but undergoes proteolytic cleavage at several pairs of basic residues that leaves its core region intact. ProSAAS is known to interact with proprotein convertase subtilisin/kexin type 1 (PCSK1) via the C-terminal inhibitory domain (aa 219-258). In the neural tissues proSAAS exhibits neuroprotective effects and is shown to block alpha-synuclein-induced cytotoxicity in cultures of dopaminergic neurons and in SH-SY5Y cells. In vitro studies have shown that proSAAS can also prevent the fibrillation of amyloid beta 1-42 peptide in a dose-dependent manner and residues 97-180 are sufficient for its anti-aggregation function against amyloid peptides. Inclusion of recombinant proSAAS in the medium of Neuro2a cells is shown to block the neurocytotoxic effect of amyloid peptide 1-42.

Specificity

This rabbit polyclonal antibody detects proSAAS in human, mouse, and rat cells. It targets an epitope within the N-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to first 180 amino acids from murine ProSAAS.

Application

Anti-proSAAS Antibody, Cat. No. ABN2268, is a highly specific rabbit polyclonal antibody that targets ProSAAS and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected proSAAS in AtT-20 cells. ( Courtesy of Dr. Iris Lindberg @ University of Maryland).
Immunofluorescence Analysis: A representative lot detected proSAAS in Immunofluorescence applications (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15; Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Guest, P.C., et. al. (2002). J Endocrinol. 175(3):637-47).
Western Blotting Analysis: A representative lot detected proSAAS in Aβ immunoprecipitated from mouse brain lysates (Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30).
Immunocytochemistry Analysis: A representative lot detected proSAAS in SH-SY5Y cells (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in His-tagged mouse proSAAS recombinant protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged mouse proSAAS recombinant protein.

Target description

~19 kDa observed; 27.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit polyclonal antiserum with 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, human, mouse. packaging: antibody small pack of 25 . μ. L. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable. isotype: IgG. UniProt accession no.: Q9QXV0. target post-translational modification: unmodified. Gene Information: human ... PCSK1N(27344).