General description
Protein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
Immunogen
Bovine
bovine liver Protein Disulfide Isomerase
Application
Immunoblotting (1:1000, colorimetric)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 50% glycerol.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, rat testis, mouse spleen
Other Notes
Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther.282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
This antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. contains: ≥. 0.9% sodium azide as preservative. species reactivity: guinea pig, Xenopus, sheep, bovine, rat, canine, hamster, porcine, mouse, monkey, human. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... PDIA2(64714). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202304
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 539229-50UL