General description
Prox1, the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1.
Specificity
Other species have not been tested.
Recognizes Prox1. The antibody is useful for identifying Prox1 expressing cells during the development of many areas of the nervous system including the dorsal spinal cord, ganglionic eminences, thalamus and hippocampus.
Immunogen
Synthetic peptide from the C-terminus of mouse Prox1.
Application
Detect Prox 1 using this Anti-Prox 1 Antibody validated for use in IC, IH.
Immunohistochemistry:
1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum.
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry
Physical form
Rabbit serum in 50 % glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Embryonic brain tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse, zebrafish. species reactivity (predicted by homology): human. manufacturer/tradename: Chemicon®. . technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable. NCBI accession no.: NM_002763.3. UniProt accession no.: Q92786. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... PROX1(5629). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5475
