ANTI-PTP ZETA/PHOSPHACAN (C15-1639-910)

Specificity

This rabbit polyclonal antibody specifically detects Phospho-NMDAR2A (Ser1459).

Immunogen

A peptide corresponding to 176 amino acids, phosphorylated on Serine 1459, from the C-terminal region of Human NMDAR2A.

Application

Quality Control Testing

Evaluated by Western Blotting in lysates from wild-type HEK293T cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NMDAR2A phosphorylated on serine 1459 in lysate from HEK293T cells, but not in lysates from cells transfected with S1459A NMDAR2A.

Tested Applications

Western Blotting Analysis: A representative lot detected phospho NMDAR2A (Ser1459) in Western Blotting applications (Wyklicky, H., et. al. (1988). Wien Klin Wochenschr. 100(7):208-10).

Immunocytochemistry Analysis: A representative lot detected phospho NMDAR2A (Ser1459) in Immunocytochemistry applications (Wyklicky, H., et. al. (1988). Wien Klin Wochenschr. 100(7):208-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Glutamate receptor ionotropic, NMDA2A (UniProt: Q12879; also known as GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, hNR2A) is encoded by the GRIN2A (also known as NMDAR2A) gene (Gene ID: 2903) in human. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2A is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off. NMDAR2A exists as a heterotetramer and the heterotetrameric channels are composed of two zeta subunits (GRIN1) and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) (in vitro). It can also form heterotetrameric channels that contain at least one zeta subunit (GRIN1), at least one epsilon subunit, plus GRIN3A or GRIN3B. In vivo, the subunit composition may depend on the expression levels of the different subunits. These heterotetrameric, ligand-gated ion channels display high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by magnesium ions. It has been shown that channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B. Mutations in GRIN2A gene are known to cause epilepsy, speech disorders, and mental retardation. Two isoforms of NMDAR2A have ben described that are produced by alternative splicing. (Ref.: Hess, SD. et al. (1996). J. Pharmacol. Exp. Therap. 278(2); 808-816).

Physical form

Purified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.