ANTI-PXDN/VPO1 (C005B-241575)

General description

Peroxidasin homolog (EC 1.11.1.7; UniProt Q92626; also known as hsPxd01, Melanoma-associated antigen MG50, p53-responsive gene 2 protein, Vascular peroxidase 1) is encoded by the PXDN (also known as COPOA, KIAA0230, MG50, PRG2, VPO, VPO1) gene (Gene ID 7837) in human. Human peroxidasin 1 (hsPxd01) belong to the superfamily of heme peroxidases with important roles in innate immunity and hormone biosynthesis, members of which also include myeloperoxidase (MPO), eosinophil peroxidase (EPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). Peroxidase 1 is a multidomain heme peroxidase that uses bromide as a cofactor in catalyzing the formation of sulfilimine bonds in collagen IV via release of hypohalous acids. Peroxidase 1 plays an essential role in tissue development and architecture by catalyzing sulfilimine bonds formation that confers critical structural reinforcement to collagen IV scaffolds. Human peroxidasin 1 is produced with a signal peptide sequence (a.a. 1-26), the remvoal of which yields the mature protein. In its N-terminal region, hsPxd01 contains four leucine-rich (LRR) repeats (a.a.87-180) flanked by two cysteine-rich domains, the LRRNT (a.a. 27-63) and LRRCT (a.a. 192-245), this is followed by four C2-type Ig-like domains, and a C-terminal von Willebrand factor type C domain (VWFC; a.a. 1413-1471) found in many secreted extracellular matrix proteins.

Specificity

This polyclonal antibody was raised against an N-terminal region sequence present in both spliced isoforms of human VPO1/PXDN protein (UniProt Q92626), but not conserved in MPO, LPO, EPO and TPO (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).

Immunogen

Epitope: LRRNT domain.

KLH-conjugated linear peptide corresponding to a LRRNT domain sequence of human PXDN/VPO1.

Application

Immunohistochemistry Analysis: A 1:400 diluttion from a representative lot detected VPO1 in mouse lung tissue sections (Courtesy of Guangjie Cheng, M.D., Emory University, GA, U.S.A.).
Immunoprecipitation Analysis: A representative lot immunoprecipitated all the radiolabelled VPO1 from the culture supernatant, but not from cell lysates, 20 hrs after an initial 20-min [32S]-Met pulse labeling of HEK293 cells stably expressing transfected VPO1 (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).
Western Blotting Analysis: A representative lots detected cellular VPO1 in total cell lysate and secreted VPO1 in concentrated culture medium following histone deacetylase inhibition by NaBu (Cat. No. 567430) treatment of HEK293 cells stably expressing exogenously transfected VPO1 (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).
Western Blotting Analysis: A representative lot detected VPO1 in human, mouse, and bovine sera samples, as well as a time-dependent cellular VPO1 upregulation in lysates from LPS- or TNF-alpha-stimulated HUVECs (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).

This Anti-PXDN Antibody is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation for the detection of PXDN.

Quality

Evaluated by Western Blotting Analysis in human plasma.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected VPO1 in 10 µg of human plasma.

Target description

>165 kDa observed. 162.4/165.3 kDa (human isoform 1 mature/pro-form), 77.50/80.33 kDa (human isoform 2 mature/pro-form), 162.4/165.1 kDa (mouse mature/pro-form) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Purified rabbit polyclonal antibody in 0.1 M Tris-glycine buffer containing 0.02% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: bovine, mouse, human. technique(s): immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_036425. UniProt accession no.: Q92626. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... PRG2(5553), PXDN(7837). Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.