Anti-Rab21 Antibody (C15-1316-746)

General description

RAB21 is a member of the small GTPase superfamily that is critically important in the recycling of integrin protein receptor complexes and their internalization. Thus, RAB21 is important in cell adhesion and migration of cells, as well as the proper completing of cytokinesis which requires particular care with adherent cells to coordinate the release and recycling of integrin and their release from the ECM matrix during cytokinesis. RAB21 is primarily a cytoplasmic or cytoplasmic organelle localized protein and its precise location will vary with cell cycle and cell division phases. RAB21 is widely expressed particularly in cells in the gut and capillaries where cell division and cell morphological changes are common.

Immunogen

GST-tagged recombinant protein corresponding to Mouse Rab21.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Rab21 Antibody is validated for use in Western Blotting for the detection of Rab21.

Western Blot Analysis: A 1:1000 dilution of a representative lot of this antibody detected Rab21 in A431, C2C12, HEK293, HeLa, HepG2, Jurkat, L6, Mouse brain, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates. This antibody did not detect Rab21 in Human placenta or Huvec cell lysates.

Western Blot Analysis: A representative lot of this antibody detected Rab21 in melanocyte membrane compartment lysate (Ohbayashi et al., (2012) Mol Biol Cell. 23(4):669-678).

Quality

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected Rab21 in 10 µg of PC12 cell lysate.

Target description

~24 kDa observed.
Uncharacterized bands may be obeserved in some lysates.

Physical form

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human, rat. technique(s): western blot: suitable. NCBI accession no.: NP_055814. UniProt accession no.: Q9UL25. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... RAB21(23011). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.