ANTI-RAD21 ANTIBODY

General description

Aka Scc1, part of the cohesin complex, Rad21 is a nuclear protein that associates with mitotic chromatin and is essential for the association of kinetochores with microtubules. Rad21/Scc1 s is cleaved by caspases, promoting apoptosis.

The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells

Specificity

Rad21

Immunogen

GST fusion protein corresponding to residues 240-631 of human Rad21

Application

Detect RAD21 with Anti-RAD21 Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Routinely evaluated by immunoblot with HeLa nuclear extracts

Target description

100-120kDa

Physical form

Format: Purified

Protein G Purified

Prtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.