Anti-RAD51 Antibody (C15-1317-040)

General description

RAD51 is essential for homologous recombination of DNA during repair of double-strand breaks. The N-terminus of RAD51 functions to bind DNA and promote homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.

Specificity

Other homologies: Bovine, Equine, and Canine (99% sequence homology).

Immunogen

Histidine-tagged recombinant protein corresponding to human RAD51.

Application

Anti-RAD51 Antibody is a Rabbit Polyclonal Antibody for detection of RAD51 also known as DNA repair protein RAD51 homolog 1, HsRAD51 & has been validated in WB, ICC & IP.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RAD51 in A431 and HeLa cells.

Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate.

Target description

~37 kDa observed. Two isoforms (isoform 1 at 37 kDa and isoform 2 at 26 kDa) produced as a result of alternative splicing are known to exist: .

Linkage

Replaces: MABE515

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse. technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_002866. UniProt accession no.: Q06609. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... RAD51(5888). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.