Anti-RalDH2 (ALDH1A2) (C15-1317-579)

General description

Retinal dehydrogenase 2 (EC 1.2.1.36; UniProt Q62148; also known as Aldehyde dehydrogenase family 1 member A2, RALDH 2, RalDH2, RALDH(II), Retinaldehyde-specific dehydrogenase type 2) is encoded by the Aldh1a2 (also known as Aldh1a7, Raldh2) gene (Gene ID 19378) in murine species. It can recognize and bind both free retinal and cellular retinol-binding protein-bound retinal and oxidize it to retinoate. The enzyme is known to be active in embryos, where retinoic acid play a crucial role in early development. RALDH2-null mice do not live beyond E10.5. The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 alpha 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA. While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs). The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns. In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina. RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera. RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE. RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.

Specificity

This polyclonal antibody targets an N-terminal region sequence of human/mouse/rat RalDH2 reported by UniProt (O94788/Q62148/Q63639).

Immunogen

KLH-conjugated linear peptide corresponding to an N-terminal region sequence of mouse RalDH2.

Application

Anti-RalDH2 (ALDH1A2) Antibody, Cat. No. ABN420, is a highly specific rabbit polyclonal antibody that targets RalDH2 (ALDH1A2) and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected RalDH2 in rat embryo tissue section.

Immunofluorescence Analysis: A 1:300 dilution from a representative lot immunostained the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistry (Courtesy of Anna Ashton, University of Aberdeen).

Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected RalDH2 in 50 µg of new born (postnatal P0) Sprague Dawley (SD) rat whole eye tissue lysate, but not in adult SD rat brain lateral cortex and pons tissue lysates (Courtesy of Anna Ashton, University of Aberdeen).

Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry in mouse retina tissue section.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected RalDH2 in mouse retina tissue section.

Target description

~57 kDa observed. 56.72/56.63/56.64 kDa (human/mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse. species reactivity (predicted by homology): canine (based on 100% sequence homology). technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_033048. UniProt accession no.: Q62148. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... ALDH1A2(8854)
mouse ... Aldh1A2(19378). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.