ANTI-RAT COLLAGEN TYPE I ANTIBODY (C15-1316-434)

General description

Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Specificity

Recognizes Rat Collagen Type I

The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Immunogen

Collagen Type I extracted and purified from rat skin.

Application

Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

RIA:
A previous lot of this antibody was used in RIA.

Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.

Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.

Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.

Quality

Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.

IHC-Paraffin Staining With Epitope Retrieval: Rat Skin

Physical form

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics

Analysis Note

Control
Rat hepatic stellate cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), radioimmunoassay: suitable. isotype: IgG. suitability: not suitable for Western blot. NCBI accession no.: NM_000088.3. UniProt accession no.: P02452. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... COL1A1(1277). Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.