General description
Renilla luciferase is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Renilla luciferase is often used as a reporter gene in sudies of gene expression or interaction. The 36 kDa enzyme catalyzes oxidation of the substrate coelenterazine to coelenteramide, resulting in the production of visible light. Renilla luciferase is not the same as luciferase from fireflies (Photalis pyralis) nor does it recognize the same substrates, so both enzymes can be used simultaneously in the same assay.
Specificity
Renilla luciferase
Immunogen
Renilla luciferase GST fusion protein
Application
Detect Renilla Luciferase using this Anti-Renilla Luciferase Antibody, clone 5B11.2 validated for use in ELISA, FUNC, NEUT & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot:
A previous lot of this antibody was used in WB.
EIA:
A previous lot of this antibody was used in ELISA.
Neutralization:
A prevous lot of this antibody was used in NEAT.
Optimal working dilutions must be determined by end user.
Target description
36 kDa
Physical form
Mouse monoclonal IgG1 Ascites.
Unpurified
Storage and Stability
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Renilla
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 41181846
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB4400
- Temperature Control Device:
- Yes
