ANTI-REPLICATION PROTEIN A (AB-1) MOUSE MAB (RPA70-9)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with the NS1 myeloma cell line. Recognizes the ~70 kDa Replication Protein A.

Recognizes the ~70 kDa subunit of replication protein A in HeLa and u293 cells and colon carcinoma tissue.

This Anti-Replication Protein A (Ab-1) Mouse mAb (RPA70-9) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Replication Protein A (Ab-1).

Immunogen

Epitope: Within the p70 subunit of replication protein A

Human

replication protein A purified from U293 cells

Application

Frozen Sections (2.5 g/ml)
Immunoblotting (1-5 g/ml)
Immunofluorescence (2.5 g/ml)
Immunoprecipitation (1 g/reaction)
Paraffin Sections (2.5 g/ml, no pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
HeLa or U293 cells or colon carcinoma tissue

Other Notes

Din, S., et al. 1990. Genes Dev.4, 968.
Brill, S.J. and Stillman, B., 1989. Nature342, 92.
Stillman, B., 1989. Annu. Rev. Cell. Biol.5, 197.
Tsurimoto, T. and Stillman, B., 1989. EMBO J.8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1834.

Specific for the p70 subunit of replication protein A. This antibody can be used as a marker for proliferation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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