ANTI-REV1 CLONE 1G9 ZOOMAB« RABBIT MONOCLONAL (C15-1669-443)

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G9 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects REV1. It targets an epitope within 22 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human REV1.

Application

Quality Control Testing

Evaluated by Western Blotting in Human kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected REV1 in Human kidney tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected REV1 in Rat Kidney tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound REV1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected REV1 in Human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected REV1 in HEK293 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

DNA repair protein REV1 (UniProt: Q9UBZ9; also known as EC:2.7.7, Alpha integrin-binding protein 80, AIBP80, Rev1-like terminal deoxycytidyl transferase) is encoded by the REV1 (also known as REV1L) gene (Gene ID: 51455) in  human. REV1 is a ubiquitous, monomeric, eukaryotic member of the Y family of DNA polymerases that is involved in the tolerance of DNA damage by translesion DNA synthesis. It serves as a deoxycytidyl transferase that transfers a dCMP residue from dCTP to the 3′-end of a DNA primer in a template-dependent reaction. It is essential for mutagenic bypass of a wide variety of lesions, a function that does not appear to be dependent on its deoxycytidyl transferase activity. REV1 interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; and this interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. It can bind directly to PCNA and monoubiquitylation of PCNA enhances this interaction. This interaction requires a functional BRCT domain (aa 44-131) located near its N-terminus. Three isoforms of REV1 have been described that are produced by alternative splicing.  This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Guo, C., et al. (2006). Mol. Cell.  23(2); 265-271; Simpson, LJ., and Sale, JE. (2003). EMBO J. 22(7); 1654-1664).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.