ANTI-RHOE/RND3 CLONE 4 100 Y56

General description

Dystroglycan (UniProt: Q28685; also known as Dystrophin-associated glycoprotein 1) is encoded by the DAG1 gene (Gene ID: 100009278) in rabbit. Dystroglycan is a component of the dystrophin glycoprotein complex that serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. Its expression is reported in the brain, cardiac muscle, skeletal muscle, and placenta. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce a-dystroglycan (aa 30-653) and b-dystroglycan (aa 654-895). a-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin-2 and 5, agrin, neurexin, and pikachurin. It is heavily O-glycosylated, which comprises of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. b-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, b-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, a/b dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant a-dystroglycan glycosylation. (Ref.: Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216; Yamada, H., et al. (1994). FEBS Lett. 352(1); 49-53; Ervasti, JM., and Campbell, KP. (1993). J. Cell Biol. 12(4); 809-823; Ibraghimov-Beskrovnaya, O., et al. (1992). Nature. 355(6362); 696-702).

Specificity

Clone IIH6 (IIH6C4) is a mouse monoclonal antibody that detects Dystroglycan (a-Dystroglycan and uncleaved form) in multiple species.

Immunogen

Rabbit skeletal muscle membrane preparation.

Application

Quality Control Testing

Evaluated by Western Blotting in Rabbit skeletal muscle tissue lysate.

Western Blotting Analysis: 1:1,000 of this antibody detected Dystroglycan in Rabbit skeletal muscle tissue lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Dystroglycan in Western Blotting applications (Azzag, K., et. al. (2020). Skelet Muscle. 10(1):10 ; Bijata, M., et. al. (2015). Front Cell Neurosci. 9:199).

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Dystroglycan in mouse heart and mouse skeletal muscle tissue sections.

Flow Cytometry Analysis: A representative lot detected Dystroglycan in Flow Cytometry applications (Gerin, I., et. al. (2016). Nat Commun. 7:11534).

Immunofluorescence Analysis: A representative lot detected Dystroglycan in Immunofluorescence applications (Azzag, K., et. al. (2020). Skelet Muscle. 10(1):10).

Inhibition Analysis: A representative lot reduced agrin-induced ERK activation in cardiac cells. (Bassat, E., et. al. (2017). Nature. 547(7662):179-184)

Immunohistochemistry Applications: A representative lot detected -Dystroglycan in Immunohistochemistry applications (Cataldi, M.P., et. al. (2018). Nat Commun. 9(1):3448).

Immunocytochemistry Analysis: A representative lot detected Dystroglycan in Immunocytochemistry applications (Bijata, M., et. al. (2015). Front Cell Neurosci. 9:199).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-a-Dystroglycan, clone IIH6C4, Cat. No. 05-593-I, is a mouse monoclonal antibody that detects Dystroglycan and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence Inhibition, Immunohistochemistry, and Western Blotting.

Physical form

Mouse monoclonal antibody without azide.

Storage and Stability

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.