ANTI-RINT-1 (C005B-240603)

General description

RAD50-interacting protein 1 (UniProt Q6NUQ1; also known as HsRINT-1, RINT-1, RAD50 interactor 1) is encoded by the RINT1 gene (Gene ID 60561) in human. Originally identified and named as a RAD50-interacting partner by a yeast two-hybrid screening, RINT1 is involved in the G2/M checkpoint, maintenance of centrosome integrity, telomere elongation, Golgi apparatus, Golgi-ER vesicular trafficking and Cis- and Trans-Golgi homeostasis. Rint1 homozygous deletion in mice causes early embryonic lethality, while Rint1 heterozygotes are predisposed to multiple tumor formation. Likewise, rare RINT1 mutations are identified to be associated with intermediate levels of breast cancer risk in human.

Specificity

This polyclonal antibody detects RINT-1 by targeting an N-terminal region sequence.

Immunogen

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding to an N-terminal sequence of human RINT-1.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected RINT-1 in A431, HeLa, HepG2, and NIH/3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-RINT-1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of RINT-1.

Quality

Evaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RINT-1 in 10 µg of MOLT-4 cell lysate.

Target description

~85 kDa observed. 90.63 kDa calculated. Target band may be resolved into a doublet with the slower migrating band representing product resulting from translation starting at an non-AUG codon (Xiao, J., et al. (2001). J. Biol. Chem. 276(9):6105-6111). Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_068749. UniProt accession no.: Q6NUQ1. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... RINT1(60561). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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