General description
Runx3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5′-PYGPYGGT-3′ found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.
Immunogen
Recombinant protein corresponding to human Runx3.
Application
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Runx3 in HCT116, SW620, and SW480 nuclear extracts. (Ito, K., et al. (2008). Cancer Cell. 14(3):226-237.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Runx3 Antibody, clone R3-5G4 (mouse monoclonal antibody) validated in WB, IP to detect Runx3 also known as Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, PEA2-alpha C, PEBP2-alpha C.
Quality
Evaluated by Western Blot in Daudi cell lysate.
Western Blot Analysis: 0.25 mg/mL of this antibody detected Runx3 in 10 µg Daudi cell lysate.
Target description
~44 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Daudi cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 41106608
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABE145
- Temperature Control Device:
- Yes
