Specificity
Ryanodine Receptor 2.
Immunogen
Synthetic peptide from the variant TM region of human Ryanodine Receptor 2.
Application
Anti-Ryanodine Receptor 2 Antibody is an antibody against Ryanodine Receptor 2 for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Signaling Neuroscience
Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
Physical form
Rabbit serum. Liquid.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human, rat. manufacturer/tradename: Chemicon®. . technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_001035.2. UniProt accession no.: Q92736. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... RYR2(6262). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51202300
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- AB9080
- Temperature Control Device:
- Yes
