ANTI-SATB2 CLONE 1H17 ZOOMAB RB MONO (C15-1670-741)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1H17 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects DNA-binding protein SATB2. It targets an epitope within 21 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human DNA-binding protein SATB2.

Application

Quality Control Testing

Evaluated by Western Blotting in K562 cell nuclear extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SATB2 in K562 cells nuclear extract.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SATB2 in Mouse and Rat brain nuclear extract.

Immunocytochemistry Analysis (ICC): A 1:100 dilution from a representative lot detected SATB2 in HeLa, A431, and HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SATB2 in human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound SATB2 peptides with a KD of 1.0 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SATB2, clone 1H17 ZooMAb^®, Cat. No. ZRB1342, is a recombinant Rabbit monoclonal antibody that specifically targets SATB2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

DNA-binding protein SATB2 (UniProt: Q9UPW6; also known as Special AT-rich sequence-binding protein 2, SATB2) is encoded by the SATB2 (also known as KIAA1034) gene (Gene ID: 23314) in human. SATB2 serves as a transcription factor controlling nuclear gene expression by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. It recognizes the sugar-phosphate structure of double-stranded DNA. It acts as a docking site for several chromatin remodeling enzymes and recruits corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. SATB2 contains two 2 CUT DNA-binding domains (aa 350-437 and 473-560) and a classic homeobox DNA binding domain (aa 615-674). It is highly expressed in the adult brain and its moderate expression has been reported in fetal brain. Postnatally, it is required for proper dendritic and soma adhesion of callosal neurons in neocortex and its deletion is reported to cause impairment in long-term memory. Mutations in SATB2 gene have been linked to cleft palate condition. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, L., et al. (2020). Cell Death Differ. 27(5); 1604-1617).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.