Anti-SDHD Antibody (C15-1316-760)

General description

Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (CybS), also known as CII-4 or QPs3 or Succinate dehydrogenase complex subunit D or Succinate-ubiquinone oxidoreductase cytochrome b small subunit or Succinate-ubiquinone reductase membrane anchor subunit, and encoded by the gene name SDHD or SD14, is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumorsuppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

Immunogen

Epitope: center region

KLH-conjugated synthetic peptide corresponding to the center region of human Anti-SDHD.

Application

Detect SDHD using this Anti-SDHD Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: 2.5 μg/mL from a representative lot detected Anti-SDHD in EL4 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in EL4 cell lysate.

Western Blotting Analysis: 2 μg/mL of this antibody detected Anti-SDHD in EL4 cell lysate.

Target description

~16 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. technique(s): immunocytochemistry: suitable, western blot: suitable. UniProt accession no.: O14521. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SDHD(6392). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.