Specificity
Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.
Immunogen
Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).
Application
Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Target description
90 kDa
Linkage
Replaces: AB5471
Physical form
Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Embryonic brain tissue, neural growth cones
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. manufacturer/tradename: Chemicon®. . technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NM_004186.2. UniProt accession no.: Q13275. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SEMA3F(6405). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51112007
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5471P
