ANTI-SEROTONIN RECEPTOR 1B CLONE 1C2.1

General description

5-hydroxytryptamine receptor 1B (UniProt P28222; also known as 5-HT-1B, 5-HT-1D-beta, 5-HT1B, S12, Serotonin 1D beta receptor, Serotonin receptor 1B) is encoded by the HTR1B (also known as HTR1DB) gene (Gene ID 3351) in human. The 5-HT receptors are grouped according to their antagonist susceptibilities and their affinities for 5-HT. The 5-HT1 receptors are a subfamily of 5-HT receptors that were originally classified according to their inhibition of adenylyl cyclase, degree of sequence similarity and their overlapping pharmacological specificities. The subfamily has five different receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT1F that can couple to Gi/Go and mediate inhibitory neurotransmission. 5HT-1B receptor belong to the family of G-protein coupled receptors that are distributed in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen. 5-HT-1B also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide. Ligand binding causes a conformation change in the receptor that triggers signaling via G-proteins, which modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling via 5-HT-1B inhibits adenylate cyclase activity. 5-HT1B receptor is known to be involved in thermoregulation, respiration, appetite control, sexual behavior, and aggression.
5-HT-1B receptor are therapeutic targets for antimigraine drugs and for treatment of bipolar disorders, autism, and anti-aggressive agents.

Specificity

Clone 1C2.1 targets an epitope within the third cytoplasmic domain of human, mouse, and rat serotonin receptor 1B.

Immunogen

GST-tagged recombinant fragment corresponding to the thrid cytoplasmic domain of human serotonin receptor 1B.

Application

Anti-Serotonin Receptor 1B, clone 1C2.1 Antibody, Cat. No. MABN1539, is a highly specific mouse monoclonal antibody that targets serotonin receptor 1B and has been tested in Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected serotonin receptor 1B in human (cerebellum and cerebral cortex) and rat (cerebral cortex and medulla) brain tissue sections.

Western Blotting Analysis: 1 µg/mL from a representative lot detected serotonin receptor 1B in 10 µg of mouse brain tissue lysate.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 1B in 10 µg of rat brain tissue lysate.

Target description

~43 kDa observed. 43.57/43.08/43.16 kDa (human/mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.