General description
5-hydroxytryptamine receptor 2A (UniProt P28223; also known as 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A) is encoded by the HTR2A (also known as HTR2) gene (Gene ID 3356) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-HT2A receptor, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action and contributes to many of the physiological effects of 5-HT. The hallucinogenic activity of LSD is mediated in part by its action as a partial to full agonist of 5-HT2A receptor, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism against 5-HT2A receptor. Human 5-HT2A receptor contains four extracellular domains (a.a. 1-75, 133-148, 216-233, 347-362), seven transmembrane segments (a.a. 76-99, 111-132, 149-171, 192-215, 234-254, 325-346, 363-384), and four cytoplasmic domains (a.a. 100-110, 172-191, 255-324, 385-471).
Specificity
Expected to react with both spliced isoforms of human Serotonin receptor 2A reported by UniProt (P28223).
Immunogen
Epitope: Third cytoplasmic domain.
GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human Serotonin receptor 2A/HTR2A.
Application
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected 2A/HTR2A in human cerebral cortex, mouse cerebral cortex, and rat testis tissue sections.
Research Category
Neuroscience
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Serotonin receptor 2A/HTR2A Antibody, clone 9B11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of SHTR2A.
Quality
Evaluated by Western Blotting in human cerebral cortex tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected 2A/HTR2A in 10 µg of human cerebral cortex tissue lysate.
Target description
~52 kDa observed. 52.60 kDa (isoform 1) and 43.94 kDa (isoform 2) calculated.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 51144602
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABN1595
- Temperature Control Device:
- Yes
