Anti-Serotonin Transporter Antibody (C15-1315-845)

Specificity

Serotonin Transporter (SERT). An antibody raised against a similar peptide has been shown to react with a SERT in baculovirus cells expressing recombinant rat and human SERT. Antibody activity may be reduced considerably when detecting endogenous SERT from rat or human cells. SERT has been observed to migrate as 94 kDa (rat platelet), 80 kDa (pulmonary membranes) and 76 kDa (rat brain). This could be due to differences in postranslational membrane modifications (e.g. glycosylation).

Immunogen

A 15 amino acid sequence between transmembrane domain 7 and 8 of the rat serotonin transporter. This region is presumably from the extracellular domain.

Application

Anti-Serotonin Transporter Antibody is an antibody against Serotonin Transporter for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Neuronal & Glial Markers

Western blot: 1-10 μg/mL


Cell pellets are resuspended in 100 μL lysis buffer (25 mM Tris, pH 7.6, 150 mM NaCl, 1 μg/mL aprotonin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate, 1 mM EDTA, and 1% Triton X-100). Samples were incubated for 1 hour at 4°C and were pelleted at high speed for 10 minutes at 4°C, and 100 μg protein per sample was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis.





ELISA: 1:100,000 primary EIA using immunogen only (50-100 ng SERT peptide (Catalog Number AG297)/well). Not tested on purified protein at the stated dilutions.


Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, western blot: suitable. NCBI accession no.: NM_001045.3. UniProt accession no.: P31645. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... HTT(3064), SLC6A4(6532). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.