General description
Serpin B5 (UniProt P36952; also known as Maspin, Peptidase inhibitor 5, PI-5) is encoded by the SERPINB5 (also known as PI5) gene (Gene ID 5268) in human. Serpin B5/Maspin is an evolutionarily conserved serine protease inhibitor that induces apoptosis and inhibits the motility of breast cancer cells. DNA hypermethylation and histone deacetylation lead to silencing of the SERPINB5 gene in human breast cancer.
Specificity
Does not detect spliced isoform 2 (UniProt P36952-2).
Immunogen
Epitope: C-terminal half
GST-tagged recombinant protein corresponding to the C-terminal of human SERPINB5.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-SERPINB5 Antibody, clone 5C6.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of SERPINB5.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected SERPINB5 in 10 µg of HT-1080 and HT-29 cell lysates.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SERPINB5 in human breast tissue.
Quality
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SERPINB5 in 10 µg of HCT116 cell lysate.
Target description
~42 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51202411
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABC603
- Temperature Control Device:
- Yes
