General description

Monoclonal Anti-SET (mouse IgG2b isotype) is derived from the hybridoma SET51 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. The SET (Suvar3-9, enhancer of zeste, trithorax) protein is localized to the nucleus and cytoplasm. It is characterized with a N-terminal domain with inhibitory activity and a highly acidic C-terminal domain that is involved in chromatin remodeling.

Immunogen

peptide corresponding to a sequence within the internal region of human SET, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-SET antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Suvar3-9, enhancer of zeste, trithorax (SET) acts as a potential inhibitor of protein phosphatase 2A (PP2A) activity. It plays a key role in the regulation of normal and cancer signal transduction. In addition, it also regulates human natural killed-cell cytotoxicity by regulating natural killer cell IFN-γ production granzyme B gene expression through PP2A inactivation. SET has an ability to inhibit the tumor suppressor NM23-H1, a granzyme A DNAse-activated factor. It is also involved in negative regulation of histone acetylation. SET- nucleoporin 214 (Nup214)/CAN fusion protein might affect the normal regulation of PP2A and leads to the development of leukemogenesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.