Anti-SF3b155 (SAP 155) Antibody (C15-1316-871)

General description

Splicing factor 3B subunit 1, also known as Pre-mRNA-splicing factor SF3b 155 kDa subunit (SF3b155), Spliceosome-associated protein 155 (SAP 155), and encoded by the gene name SF3B1 or SAP155, is a member of the SF3B1 family . SF3B155 (SAP 155) functions a s a subunit of the splicing factor SF3B required for ′A′ complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B155 (SAP 155) has been implied to be involved in the assembly of the ′E′ complex, and also may anchor U2 snRNP to the pre-mRNA. Additionally, SF3B155 (SAP 155) belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. SF3B155 (SAP 155) has been identified in the spliceosome C complex, as a component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Phosphorylation of SF3B155 (SAP 155) occurs concomitantly with the splicing catalytic steps. The phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. SF3B155 (SAP 155) is located in the nucleus speckle and during mitosis, it is transiently dispersed from the nuclear speckles to the cytoplasm.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human SF3b155 (SAP 155) near the N-terminus.

Application

Anti-SF3b155 (SAP 155) is an antibody against SF3b155 (SAP 155) for use in WB, ICC.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SF3b155 (SAP 155) in HeLa, A431, and NIH/3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SF3b155 (SAP 155) in 10 µg of HeLa cell lysate.

Target description

~160 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human, rat. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_036565. UniProt accession no.: O75533. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SF3B1(23451). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.