ANTI-SHOC2 ANTIBODY (C15-1317-887)

General description

The Leucine-rich repeat protein SHOC-2 (SHOC2) is the regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and plays a key role in MAPK pathway activation. Upon RAS activation, SHOC2 acts as a positive modulator of the RAS-MAPK signaling via specifically allowing PP1c to dephosphorylate the ′Ser-259′ inhibitory site of RAF1 kinase and stimulate RAF1 activity. SHOC2 localizes to cytoplasm when inactive but rapidly translocates to the nucleus when activated, such as during growth factor stimulation. Thus, as expected, SHOC2 also participates in FGF receptor signaling. Molecularly, SHOC2 is a scaffold protein that brings together activated Ras (activated via G-protein coupled receptors and the interaction of GTP to GDP) and Raf, which then facilitates the transfer of the phospho-Raf phosphate to other proteins for the activation of the MAP Kinase pathway for gene activation. Defects in SHOC2 lead to a type of multi-symptomatic disorder termed the Noonan Syndrome Loss of Anagen Hair (NSLAH) that affects everything from skeletal formation to facial features and many organs including the brain, skin, muscles, and heart. EMD Millipore’s Anti-SHOC2 rabbit polyclonal antibody has been tested in western blotting and immunohistochemistry in human samples.

Immunogen

KLH-conjugated linear peptide corresponding to human SHOC2.

Application

Detect Leucine-rich repeat protein SHOC-2 using this rabbit polyclonal antibody, Anti-SHOC2 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected SHOC2 in human spleen tissue.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SHOC2 in 15 µg of Jurkat cell lysate.

Target description

~65 kDa observed. Uniprot describes 2 isoforms at ~60 kDa and ~65 kDa Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q9UQ13. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SHOC2(8036). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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