ANTI-SIGLEC-7/CD328 CLONE 1B10 ZOOMAB RABBIT MONOCLONAL (C15-1671-227)

General description

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ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Sialic acid-binding Ig-like lectin-7 (Siglec-7/CD328). It targets an epitope within 20 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal, cytoplasmic domain of human Sialic acid-binding Ig-like lectin 7 (Siglec-7/CD328).

Application

Anti-Siglec-7/CD328, clone 1B10 ZooMAb, Cat. No. ZRB1030, is a Rabbit monoclonal antibody that detects Siglec-7/CD328 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Siglec-7/CD328 in Jurkat and HL-60 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Siglec-7/CD328 in Raw264.7 cells.

Affinity Binding Assay: A representative lot of this antibody bound Siglec-7 peptide peptide with a KD of 3.6 x 10-9 in an affinity binding assay.

Flow Cytometry Analysis: 1 μg from a representative lot detected Siglec-7/CD328 in one million Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Siglec-7/CD328 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Sialic acid-binding Ig-like lectin 7 (UniProt: Q9Y286; also known as Siglec-7 Adhesion inhibitory receptor molecule 1, AIRM-1, CDw328, D-siglec, QA79 membrane protein, p75, CD328) is encoded by the SIGLEC7 (also known as AIRM1) gene (Gene ID: 27036) in human. Siglec-7 is a single-pass type I membrane glycoprotein protein that is predominantly expressed by resting and activated natural killer (NK) cells. Its high expression is also observed in placenta, liver, lung, spleen, and peripheral blood leukocytes. Siglec-7 serves as an inhibitory receptor expressed on NK cells. Siglec-7 is also expressed on β-cells where it is shown to restore β-cell function and survival and reduce inflammation in pancreatic islets from patients with diabetes. Its levels are down-regulated in Type 1 and Type 2 diabetes. It preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid and also binds disialogangliosides. Siglec-7 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that has an extracellular domain (aa 19-353), a transmembrane domain (aa 354-376), and a cytoplasmic domain (aa 377-467). Siglec-7 also contains one copy of immunoreceptor tyrosine-based inhibitor motif (ITIM) that is involved in modulation its cellular responses. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shao, JY., et al. (2016). Scand. J. Immunol. 84(3); 182-190; Dharmadhikari, G., et al. (2017). Sci. Rep. 7; 45319).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.