ANTI-SIRT1(SIR2) ALEXA FLUOR(R)488 CONJ

General description

NAD-dependent protein deacetylase sirtuin-1 (UniProt Q923E4; also known as Sir2, mSIRT2a, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID 93759) in murine species. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms. Sirtuins have been implicated in the regulation of aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) & aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity and cellular functions. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 & pS47 targeted by CaMKKβ.

Specificity

This antibody recognizes Sirt1.

Immunogen

Epitope: a.a. 1-131

GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sirt1(Sir2) in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-131) is shown to be suitable also for Western blotting, immunoprecipitation, and ChIP applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Sirt1 (Sir2) Antibody, Alexa Fluor^™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Sirt1 (Sir2).

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sirt1(Sir2) in A431 cells.

Target description

~110 kDa observed

Physical form

Affinity purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.