General description

Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.

Immunogen

KLH-conjugated linear peptide corresponding to human Sirt5.

Application

Immunohistochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected Sirt5 in convoluted tubules of normal rat kidney tissue.

Use Anti-Sirt5 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Sirt5 also known as NAD-dependent deacetylase sirtuin-5, SIR2-like protein 5.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Sirt5 on 10 µg of HeLa cell lysate.

Target description

~34 kDa observed. Two isoforms at ~33 and ~34 kDa are known to exist.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_036373. UniProt accession no.: Q9NXA8. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SIRT5(23408). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.