ANTI-SKP1 CLONE 3F3.1

General description

S-phase kinase-associated protein 1 (UniProt P63208; also known as Cyclin-A/CDK2-associated protein p19, OCP-2, OCP-II, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like) is encoded by the SKP1 (also known as EMC19, OCP2, SKP1A, TCEB1L) gene (Gene ID 6500) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Skp1 is one of the three core components of the SCF complex (Skp1, Cullin, F-box) and functions as an adapter that bridges the F-box protein and Cullin (CUL1). SCF complex depends on various F-box proteins for specific recognition and recruitment of substrates. SCF ligase plays an important role in regulating the transitions between G1/S and G2/M phases.

Specificity

Expected to react with both spliced isoforms of human SKP1 reported by UniProt (P63208).

Immunogen

Epitope: Internal.

GST-tagged recombinant human SKP1 internal fragment.

Application

Anti-SKP1 Antibody, clone 3F3.1 is an antibody against SKP1 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected SKP1 in HUVECs, A431 and HeLa cells.

Research Category
Signaling

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SKP1 in 10 µg of NIH/3T3 cell lysate.

Target description

~18 kDa observed. 18.66 kDa (Isoform 1) and 18.06 kDa (Isoform 2) calculated.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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