Anti-Slit2 Antibody, C-term (C15-1316-331)

General description

Slit homolog 2 protein (UniProt O94813; also known as Slit-2, Slit2) is encoded by the SLIT2 (also known as SLIL3) gene (Gene ID 9353) in human. Slit proteins are essential for midline guidance in the forebrain where they act as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Slit-2 acts as molecular guidance cue in cellular migration and its function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. Ref.: Niclou, SP., et al. (2000) J Neurosci. 20; 4962-4974.

Specificity

This polyclonal antibody targets region within the CTCK domain (a.a. 1453-1528) ane will therefore react with uncleaved Slit-2 and cleaved Slit-2 C-product (a.a. 1122-1529), but not the cleaved N-product (a.a. 31-1121).

Immunogen

KLH-conjugated linear peptide corresponding to a sequence within the CTCK domain of human Slit-2.

Application

Anti-Slit2, C-term, Cat. No. AB5701-I, is a highly specific rabbit polyclonal antibody that targets Slit2 and has been tested in Western Blotting.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Slit-2 in 10 µg of U2OS cell lysate.

Target description

~180 kDa observed. 166.8/166.3/165.8 kDa (isoform 1/2/3 without signal peptide a.a. 1-30) and 44.52 kDa (C-product) calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB5701

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_004778. UniProt accession no.: O94813. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... SLIT2(9353). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.