General description
The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Specificity
Detects Smac/DIABLO in an approximately 25 kDa band by western blot.
Immunogen
Peptide corresponding to aa 225-239 (EERAESEQEAYLRED) of human Smac/DIABLO.
Application
This Anti-Smac/Diablo Antibody is validated for use in WB for the detection of Smac/Diablo.
Western blot: 1:500 to 1:1000
Human heart tissue lysate can be used as a positive control.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse, rat. manufacturer/tradename: Chemicon®. . technique(s): western blot: suitable. NCBI accession no.: NM_019887.3, NM_138929.2, NM_138930.2. UniProt accession no.: Q9NR28. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DIABLO(56616). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB3609
