General description
Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.
Specificity
Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity.
Immunogen
Epitope: N-terminal half
Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).
Application
Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Quality
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.
Target description
~25 kDa observed
Linkage
Replaces: MAB331
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51173210
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB331-C
- Temperature Control Device:
- Yes
