ANTI-SNON ANTIBODY (C005B-240620)

General description

The Ski family of oncogenes includes Ski and Sno (Ski-related novel gene, or Ski-like). Ski family members are nuclear proteins that form homodimers and heterodimers, bind to DNA and function as transcriptional activators and repressors. The Ski proteins regulate TGFß induced gene-specific transcriptional activation by effectively repressing Smad activity and, thereby, inhibit TGFß induced cell growth and extracellular matrix production. Recently, SnoN has been shown to maintain pluripotency through selective repression of mesendodermal genes in human ES cells.

Immunogen

KLH-conjugated linear peptide corresponding to amino acids 358-372 of human SnoN protein.

Application

Immunohistochemical Analysis: A 1:50 dilution of this antibody detected SnoN in human skeletal muscle myocytes. Immunohistochemical Analysis: A 1:50 dilution of this antibody detected SnoN in human lung tissue.
Immunocytochemicaly Analysis: A 1:100 dilution of this antibody detected SnoN in H9 human embryonic stem cells.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-SnoN Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of SnoN.

Quality

Evaluated by Western Blot in a human placenta tissue lysate. Western Blot Analysis: 1 μg/ml of this antibody detected SnoN in a 10μg of human placenta tissue lysate.

Target description

~80 kDa observed

Physical form

Antigen Affinity Purified

Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_005405. UniProt accession no.: P12757. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SKIL(6498). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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