Anti-SNX18 Antibody (C15-1316-785)

General description

Sorting nexin-18 (UniProt Q96RF0; also known as SH3 and PX domain-containing protein 3B, Sorting nexin-associated Golgi protein 1) is encoded by the SNX18 (also known as SNAG1, SH3PX2, SH3PXD3B) gene (Gene ID 112574) in human. SNX18, SNX33, and SNX9 constitute a subfamily of sorting nexin (SNX) proteins characterized by a Bin–Amphiphysin–Rvs (BAR) domain that allows dimerization. It also contributes to modulation and shaping of membrane curvature. It also has a Src-homology 3 (SH3) domain that allows interaction with a wide range of proline-rich PXXP motif containing proteins. In addition to mediating endocytosis and intracellular vesicle trafficking, SNX18, SNX33, and SNX9 are also essential for successful completion of the ingression and abscission stages of cytokinesis and for the delivery of a subset of vesicles to the intracellular bridge that connects the two nascent daughter cells during mitosis. GSK‐3 is reported to prevent FIP5-SNX18 interaction during metaphase and anaphase in Madin‐Darby canine kidney (MDCK) cells by catalyzing FIP5 Thr276 phosphorylation to ensure the fidelity and timing of FIP5‐endosome targeting to the AMIS during apical lumen formation.

Specificity

Expected to react with all spliced isoforms.

Immunogen

Recombinant protein corresponding to human SNX18.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-SNX18 Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of SNX18 .

Western Blotting Analysis: A representative lot detected endogenous SNX18 in MDCK and HeLa cell lysates (Willenborg, C., et al. (2011). J Cell Biol. 195(1):71-86).
Western Blotting Analysis: A representative lot detected endogenous SNX18 in MDCK cell lysates (Li, D., et al. (2014). EMBO J. 15(4):428-437).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous SNX18 in MDCK cell lysates (Willenborg, C., et al. (2011). J Cell Biol. 195(1):71-86).
Immunocytochemistry Analysis: A representative lot detected SNX18 in filter-grown, polarized MDCK cells, as well as polarized MDCK cells grown in 3D cultures (Willenborg, C., et al. (2011). J Cell Biol. 195(1):71-86).

Quality

Evaluated by Western Blotting in MDCK cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNX18 in 10 µg of MDCK cell lysate.

Target description

~73 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, canine. technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_001096045. UniProt accession no.: Q96RF0. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... SNX18(112574). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.