ANTI-SNX9 CLONE 6C6 (C15-1885-017)

General description

Dystroglycan (UniProt: Q14118; also known as Dystrophin-associated glycoprotein 1) is encoded by the DAG1 gene (Gene ID: 1605) in human. The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. It also serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. Its expression is reported in a variety of fetal and adult tissues. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce -dystroglycan (aa 30-653) and β-dystroglycan (aa 654-895). -dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin-2 and 5, agrin, neurexin, and pikachurin. It is heavily O-glycosylated, which comprises of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. It plays important roles in the deposition, organization, and stability of basement membranes. β-dystroglycan is a single-pass type 1 transmembrane protein with its cytoplasmic tail bound to utrophin or dystrophin. It plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, β-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, /β- dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant -dystroglycan glycosylation. (Ref.: Alonso-Rangel, L., et al. (2017). Life Sci. 182; 1-9; Yoon, JH., et al. (2012). J Proteome Res. 11(9); 4413-4424).

Specificity

Clone 7D11 is a mouse monoclonal antibody that detects β-Dystroglycan. It targets an epitope within 15 amino acids from the C-terminal region.

Immunogen

A linear peptide corresponding to 15 amino acids from the C-terminal region of human β-Dystroglycan.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected β-Dystroglycan in HeLa cell lysates.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected β-Dystroglycan in Immunocytochemistry applications (Azuara-Medina, P.M., et al. (2019). Cell Death Dis. 10(3):196).

Western Blotting Analysis: A representative lot detected β-Dystroglycan in Western Blotting applications (Sherman, D.L., et al. (2001). Neuron. 30(3):677-87; Acharyya, S., et al. (2005). Cancer Cell. 8(5):421-32; Azuara-Medina, P.M., et al. (2019). Cell Death Dis. 10(3):196; Alonso-Rangel, L., et al. (2017). Life Sci. 182:1-9).

Immunofluorescence Analysis: A representative lot detected β-Dystroglycan in Immunofluorescence applications (Sherman, D.L., et al. (2001). Neuron. 30(3):677-87; Acharyya, S., et al. (2005). Cancer Cell. 8(5):421-32; Azuara-Medina, P.M., et al. (2019). Cell Death Dis.10(3):196; Moretti, A., et al. (2020). Nat Med. 26(2):207-214).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-β-Dystroglycan, clone 7D11, Cat. No. MABT1546, is a mouse monoclonal antibody that detects β-Dystroglycan and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.