ANTI-SOD1 (ALS MUTANT ) CLONE MS785 (MOUSE MONOCLONAL)

General description

SOD1 or Superoxide dismutase 1 is a critical enzyme for free radical metabolism and serves as a major detoxifier in cellular metabolism. SOD1 mutations are associated with disease and in particular with Amyotrophic lateral sclerosis 1, or ALS, often referred to as “Lou Gehrig’s Disease.” Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is characterized by the selective loss of upper and lower motor neurons. There are >100 mutations in SOD1 in humans but not all appear to be pathogenic. The clone MS785 is a monoclonal antibody generated against the Derlin-1-binding region of SOD1 (DBR), and it distinguished most ALS-causative SOD1 mutants from both wild-type and nontoxic mutants. Moreover, MS785 recognized endogenous SOD1 in B lymphocytes derived from 14 ALS patients carrying SOD1 mutations but not from 11 healthy controls, thus MS785 appears to selectively recognize all Derlin-1–interactive SOD1 mutants but not WT SOD1.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human SOD1.

Application

Detect Anti-SOD1 Antibody (ALS mutant ), clone MS785 using this Anti-SOD1 Antibody (ALS mutant ), clone MS785 validated for use in Western Blotting
and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot immunoprecipitated SOD1 in transfected HEK293 cells (Fujisawa, T., et al. (2012). Ann Neurol. 72: 739–749).
Western Blotting Analysis: A representative lot SOD1 in transfected HEK293 cells (Fujisawa, T., et al. (2012). Ann Neurol. 72: 739–749).
Immunoprecipitation Analysis: A representative lot immunoprecipitated SOD1 in transfected HEK293 cells (Homma, K., et al. (2013). Ann Neurol. Cell. 52:1-12).
Immunocytochemistry (ICC): A representative lot of this antibody detected SOD1-G93A in HEK293 cells overexpressing G93A, but not the wild-type SOD1. (Fujisawa, T., et al. 2015. Neurobiol. Dis. 82; 486-487).
Immunohistochemistry (IHC): A representative lot of this antibody detected SOD1-G93A in the anterior horns of spinal cord tissue sections of SOD1G93A-transgenic mice, but not non-transgenic mice. ((Fujisawa, T., et al. 2015. Neurobiol. Dis. 82; 486-487).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in transfected HEK293 with SOD1 mutant and HEK293 control lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SOD1 in 1 µg of transfected HEK293 with SOD1 mutant and HEK293 control lysates.

Target description

~16 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.