ANTI-SODIUM CHANNEL NAB2 ANTIBODY (C15-1316-229)

Specificity

Recognizes Nab2. The epitope specific for Nab2 is not present in any other known proteins.

Immunogen

Highly purified peptide corresponding to residues 197-215 of rat beta2 subunit of voltage-gated Na+ channels (Isom et al. 1995). Accession P54900. It is expected to work on human because the immunogen sequence is highly conserved (17/19 residues). Reactivity with other species has not yet been tested.

Application

Anti-Sodium Channel NaB2 Antibody detects level of Sodium Channel NaB2 & has been published & validated for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blotting: 1:200 on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.05 % sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_004588.3. UniProt accession no.: O60939. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SCN2B(6327). Hazard Statements: H412. Precautionary Statements: P273 - P501. Hazard Classifications: Aquatic Chronic 3. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3.